1.Two scientists are performing a test to measure the concentration…1.Two scientists are performing a test to measure the concentration of antibodies against HIV in a patient sample using competitive radioimmunoassay technique.After performing the procedure,scientist A measured the labelled radioactive agent in supernatant and scientist B used the sediment.(a)Explain the term competitive radioimmunoassay.(b)Explain and interpret the results of both Scientist A and Scientist B(c)Give an example of a radioactive agent(d)Give one advantage and one disadvantage of the used technique 2.Using HIV as an example,diacuss indirect sandwich ELISA.Giving names of all your reagents. 3.With the aid of diagrams,Explain Ouchterlony of partial identity and Ouchterlony of identity. 4(a)Compare and contrast precipitation and agglutination. (b)Write short notes on haemoagglutination. 5.(a)Discuss complement fixation technique. (b)Outline the reaction procedure for an infection positive or negative result using the complement fixation technique. (c)Discuss fluorescence flow cytometry. 6.When a microphone is used for agglutination studies there is no centrifugation to confirm the results. With the aid of a diagram,Explain how a positive and negative result is seen. BiologyScienceMicrobiology BIOLOGICAL 210
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