solved: here are my answers for my experiment can you choose from this…

  

QuestionAnswered step-by-stephere are my answers for my experiment can you choose from this…here are my answers for my experiment can you choose from this excel sheet what my unknown bacterial species is thisthis is my result Test Result (highlight/bold your result for each test) Explanation/rationale for how came up with your result. For example, if you said your unknown is amylose positive, then explain how you arrived at that conclusion based on the appearance of the test result.Gram reaction PositiveNegative This unknown bacteria is gram negative rod. Because in case of gram staining in red or pink colored bacteria’s are referred as gram negative (stained with the stain safranin) while the purple or blue colored bacteria’s are referred as gram positive (stained with the stain crystal violet)Morphology Coccus Bacillus This organisms are rod shaped so we can referred as Bacillus.  if the organisms are circular in shape then it can referred as coccus.Arrangement MonoStaphyloStrepto This bacteria are present singly so we can referred as mono. if the bacteria are present in cluster like appearance, then it is called as staphylo and if it is present in chain like appearance then it is called as strepto.Amylose PositiveNegative Zone of inhibition (clear zone around the bacterial colony) is not present around the bacterial colony so the amylose test for this bacteria is negative. Casein PositiveNegative Zone of hydrolysis (clear zone around the bacterial colony) is present around the bacterial colony so the casein test for this bacteria is positive. Indole PositiveNegative This unknown bacteria is indole negative because no red colored ring is observed onto the top of the tube. after addition of Kovac’s reagent if red or pink colored ring is observed onto the top of the tube then this test is positive.Urea PositiveNegative This unknown bacteria is urea negative because no red or pink colored growth is observed onto the top or slanting position of the tube. after incubation, if the pink or red colored is observed on the slant then this test is positive.Catalase PositiveNegative This bacteria is catalase positive because effervescence or bubbles is observed on the slide. if the bacteria is catalase positive then the bubbles are observed when the bacterial culture is inoculated in the hydrogen peroxide. Glucose Durham tube Choose all that apply:PositiveNegativeProduced acidProduced gasProduced alkaline products This unknown bacteria is positive for glucose fermentation. when we inoculate the culture in glucose fermentation broth, after incubation the red color is turn into the yellow color that means this organism is ferment the glucose sugar so it is glucose Durham tube positive. and also the gas bubble present in the Durham’s tube that indicates the bacteria having ability to produce gas. Lactose Durham tube Choose all that apply:PositiveNegativeProduced acidProduced gasProduced alkaline products This unknown bacteria is negative for lactose fermentation. when we inoculate the culture in lactose fermentation broth, after incubation the red color is remains same that means this organism is not able to ferment the lactose sugar so it is lactose Durham tube negative. and also the little amount of gas bubble present in the Durham’s tube that indicates the bacteria having ability to produce gas. Mannitol Durham tube Choose all that apply:PositiveNegativeProduced acidProduced gasProduced alkaline products This unknown bacteria is positive for mannitol fermentation. when we inoculate the culture in mannitol fermentation broth, after incubation the red color is turn into the yellow color that means this organism is ferment the mannitol sugar so it is mannitol Durham tube positive. and also the gas bubble present in the Durham’s tube that indicates the bacteria having ability to produce acid as well as gas. Sucrose Durham tube Choose all that apply:PositiveNegativeProduced acidProduced gasProduced alkaline products This unknown bacteria is negative for sucrose fermentation. when we inoculate the culture in sucrose fermentation broth, after incubation the red color is remains same that means this organism is not able to ferment the sucrose sugar so it is sucrose Durham tube negative. and also the little amount of gas bubble present in the Durham’s tube that indicates the bacteria having ability to produce gas. Methyl red PositiveNegative Red colored broth is observed after inoculation and incubation of given unknown bacteria, so the methyl red test for this bacteria is positive.if the tube is yellow in its color then the test is negative. Vogues-Proskauer PositiveNegative Red colored broth is not observed after inoculation and incubation of given unknown bacteria, so the Vogues-Proskauer test for this bacteria is negative.if the tube is red at the top side then this test is positive. Nitrate PositiveNegative Bacterial growth is observed in this test tubes or broth but the nitrate tube is negative for this unknown bacteria because no red color is observed in this broth after addition of reagents. if the red color is observed in the broth then this test is positive for the same.Citrate PositiveNegative Blue color is observed onto the slant after incubation that means the citrate test for this unknown bacteria is positive. green color is turn into the blue color. if the green color remains same then the citrate test is negative. Motility PositiveNegative Bacterial growth is observed in all over the tube that means the bacteria’s having motility (ability to move from one site to other site with the help of flagella).Description of growth on TSA plate Type your response in the next column over à Tryptic soy agar (TSA) is the starting or initial growth medium for cultivation of unknown organisms. white colored circular bacterial colonies are observed on the TSA agar plate. having entire margin and opaque in its nature. can you tell me what my unknoiwn bacterial species is can you choose from this table based on my result aboveSpecies ATCC # Gram Rxn Cell Morph. Arrange-ment2 Endo- Acid Capsule Colony  Amylose Casein Indole Urease     Durham Fermentation (48h)5           TSI, 24-36h    spores Fast Pigment3 Hydrolysis Hydrolysis (tryptophan) Catalase Oxidase4 Glucose Lactose Mannitol Sucrose MSA6 MR VP Nitrate Citrate  (slant/ butt)5 Motility Hemolysis7Alcaligenes faecalis 8750 – Bacillus Mono- – – – Beige/pinkish – – – – + + NR NR NR NR – – – – + K / NR – AlphaBacillus cereus 11778 + Bacillus Strepto- + – + Cream à white + + – -/+ + + A NR NR A – + + + – K / A + BetaBacillus megaterium 14581 + Bacillus Strepto- + – – Off-white (+) + – + + + A NR NR NR +F – – – – A / A – AlphaBacillus subtilis 6051 + Bacillus Strepto- + – – Cream + + – + + + A K A A +F – + + + K / A + Slow BetaCitrobacter freundii NCTC9750 – Bacillus Mono- – – + Cream – – – + + – AG AG AG AG – + – + + A/ A – AlphaCorynebacterium xerosis 373 + Bacillus Mono- – – – Cream/yellow  – – – – + – NR NR NR NR + – – – – K / NR – AlphaEnterobacter aerogenes 13048 – Bacillus Mono- – – – Beige/Tan – – – – + + AG AG AG AG – – + + + A / A(G) + AlphaEnterococcus durans MLT + Coccus1 Strepto- – – + Translucent – – – – – – A A NR K – + +/- – – A / A – BetaEnterococcus faecalis 29212 + Coccus1 Strepto- – – – Translucent – – – – – – A A A A – + – – – A / A – AlphaEscherichia coli 25922 – Bacillus Mono- – – +/- Beige – – + – + + AG AG AG K – + – + – A / AG + GammaKlebsiella pneumoniae 13882 – Bacillus Mono- – – + Cream – – – + + + AG AG AG AG (+) + – + + A / AG – AlphaMicrococcus luteus 4698 + Coccus Sarcina – – – Bright yellow – – – + + + NR NR NR NR – – – – – K / NR – AlphaMicrococcus roseus BSL1 + Coccus Sarcina – – + Salmon – – – – + – NR NR NR NR – – – + – K / NR – GammaMoraxella catarrhalis 8176 – Coccus Diplo- – – + Transl. white – – – + + + NR NR NR NR – – – – – K / NR (+) GammaMycobacterium smegmatis 144468 + Bacillus Mono- – + – Cream – (+) – + + – NR NR NR NR – – – + – K / NR – GammaNeisseria sicca 9913 – Coccus Diplo- – – – White/beige – – – – + + NR NR NR NR – – – – – K / NR – GammaProteus mirabilis 12453 – Bacillus Mono- – – + Transparent  – + – + + + AG K K NR (+) + – + + K /H2S A + Slow BetaProteus vulgaris 8427 – Bacillus Mono- – – – Tan, swarms – + + + + (+) AG NR NR AG – + – + – A /H2S A + BetaPseudomonas aeruginosa 10145 – Bacillus Mono- – – – Beige; grn agar + + – + + + K K K K – – – + + K H2S/ K – BetaSalmonella typhimurium 14023 – Bacillus Mono- – – + Cream / Beige – – – – + + AG K AG K +F + – + + K /H2S A + AlphaSerratia marcescens BSL1 – Bacillus Mono- – – + Deep red – – – – + + A NR A A – – + + + A / A + AlphaStaphylococcus aureus 25923 + Coccus Staph – – – Creamy yellow – – – + + – A A A A +F + + + – A / A – BetaStaphylococcus epidermidis 12223 + Coccus Staph – – – Gray-white – – – (+) + – A A (A) A + + – + – A / A – Slow BetaStreptococcus salivarius 13419 + Coccus Strepto- – – + White – –  – – – – A A NR A – + – –  – A / A –  AlphaBiologyScienceMicrobiologyBIOL 260Share Question

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